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unoq q1 column  (Bio-Rad)


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    Bio-Rad unoq q1 column
    Unoq Q1 Column, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/unoq q1 column/product/Bio-Rad
    Average 90 stars, based on 1 article reviews
    unoq q1 column - by Bioz Stars, 2026-02
    90/100 stars

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    FIG. 3. Fast Protein Liquid Chromatography separation of cal- pain isoforms on a 1 ml <t>UnoQ</t> (Bio-Rad) quaternary ion ex- change column. The earlier peak contained m-Bam-C115S--DraI-m- 80k/NHis1021k calpain, and the later peak contained C105S-m-80k/21k calpain. The column was run in 50 mM Tris-HCl, pH 7.6, 2 mM EDTA, 0.1% (v/v) Triton X-100, 10 mM 2-mercaptoethanol (buffer A) with a gradient of increasing NaCl concentration. a, a mixture of the <t>two</t> <t>proteins</t> resolved without previous exposure to Ca2. b, a mixture of the two proteins resolved following exposure to 5 mM Ca2 for 30 min, quenching with excess EDTA, and dialysis overnight against buffer A.
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    FIG. 3. Fast Protein Liquid Chromatography separation of cal- pain isoforms on a 1 ml UnoQ (Bio-Rad) quaternary ion ex- change column. The earlier peak contained m-Bam-C115S--DraI-m- 80k/NHis1021k calpain, and the later peak contained C105S-m-80k/21k calpain. The column was run in 50 mM Tris-HCl, pH 7.6, 2 mM EDTA, 0.1% (v/v) Triton X-100, 10 mM 2-mercaptoethanol (buffer A) with a gradient of increasing NaCl concentration. a, a mixture of the two proteins resolved without previous exposure to Ca2. b, a mixture of the two proteins resolved following exposure to 5 mM Ca2 for 30 min, quenching with excess EDTA, and dialysis overnight against buffer A.

    Journal: Journal of Biological Chemistry

    Article Title: Dissociation and Aggregation of Calpain in the Presence of Calcium

    doi: 10.1074/jbc.m105149200

    Figure Lengend Snippet: FIG. 3. Fast Protein Liquid Chromatography separation of cal- pain isoforms on a 1 ml UnoQ (Bio-Rad) quaternary ion ex- change column. The earlier peak contained m-Bam-C115S--DraI-m- 80k/NHis1021k calpain, and the later peak contained C105S-m-80k/21k calpain. The column was run in 50 mM Tris-HCl, pH 7.6, 2 mM EDTA, 0.1% (v/v) Triton X-100, 10 mM 2-mercaptoethanol (buffer A) with a gradient of increasing NaCl concentration. a, a mixture of the two proteins resolved without previous exposure to Ca2. b, a mixture of the two proteins resolved following exposure to 5 mM Ca2 for 30 min, quenching with excess EDTA, and dialysis overnight against buffer A.

    Article Snippet: Equal amounts (0.75 mg each) of the two proteins were mixed and resolved by chromatography on a Bio-Rad UnoQ (1 ml) ion exchange column with a gradient of [NaCl] in buffer A.

    Techniques: Fast Protein Liquid Chromatography, Concentration Assay